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ASCP ASCP-MLT Exam Syllabus Topics:

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Either immunofixation or immunoelectrophoresis can be used to confirm Bence-Jones proteinuria.
Which of the following methods would be used to confirm the presence of Bence-Jones protein in the urine:

A) Isoelectric focusing
B) Urine protein electrophoresis
C) Sulfosalicylic acid
D) Immunofixation

2. The patient's BUN is within normal range (5-20 mg/dL) while the creatinine is about five times the upper normal range (0.6-1.2 mg/dL). Gross elevations in creatinine are almost always accompanied by elevations in BUN when there is kidney impairment. Either the BUN or creatinine value in this case is incorrect. Both tests should be repeated.
A patient's BUN value is 15 mg/dl and his creatinine is 5 mg/dl. If this patient is not undergoing dialysis, what conclusion would you draw from these results?

A) patient is in early stage of renal disease
B) one of the values is in error
C) patient is normal
D) patient has suffered muscle deterioration
E) patient protein intake is quite low

3. Ammonium biurate crystalsare typically round, irregularly spiked and yellow-brown in color.
A microscopic examination of a normal urine pH 8.0 shows 2+ yellow-brown thorny spheres which are MOST probably:

A) ammonium biurate crystals
B) crenated red cells
C) amorphous urate crystals
D) ampicillin crystals
E) waxy casts

4. Assuming an alpha hemolytic reaction (not well seen in the image), viridans streptococcus and S. pneumoniae are the two possible responses. However, these colonies are far too mucoid for viridans streptococci; therefore,
S. pneumoniae is the most likely choice. Also, the colonies are much too large and the hemolytic reaction is wrong for S. pyogenes or S. agalactiae.
A patient was admitted to the hospital recently with an obvious infection. A sputum specimen was submitted and the microbiologist inoculated it to sheep blood agar. Based on the colony morphology and the alpha hemolysis seen in the image to the right, the most likely identification is:

A) Streptococcus pneumoniae
B) viridans streptococcus
C) Streptococcus agalactiae
D) Streptococcus pyogenes

5. In radioimmunassay, a fixed concentration of labeled antigen is incubated with a constant amount of antiserum such that the concentration of antigen binding sites on the antibody is limiting. If unlabeled antigen is added to this system, there is competition between labeled antigen and unlabeled antigen for the limited and constant number of binding sites on the antibody, and thus the amount of labeled antigen bound to antibody will decrease as the concentration of unlabeled antigen increases.
Which of the following choices is correct when describing the principles of competitive radioimmunoassay procedures?

A) antibody will react equally with labeled and unlabeled antigen
B) antibody will react preferentially with the unlabeled antigen
C) antibody will react directly with radioactive labeled immunoglobulin
D) antibody will react preferentially with the labeled antigen
E) antibody will react with labeled antigen only

Solutions: